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Combining MeDIP-seq and MRE-seq to estimate single CpG methylation genome wide.

flashw简单加速器-快连加速器app

No current assay of DNA methylation provides single-CpG resolution, comprehensive genome-wide coverage, and cost feasibility for a typical laboratory. To address this gap, we introduce methylCRF, a novel Conditional Random Field-based algorithm that integrates methylated DNA immunoprecipitation (MeDIP-seq) and methylation-sensitive restriction enzyme (MRE-seq) sequencing data to predict DNA methylation levels at single CpG resolution. Our method is a combined computational and experimental strategy to produce DNA methylomes of all 28 million CpGs in the human genome for a fraction (<10%) of the cost of whole genome bisulfite sequencing methods.

methylCRF was benchmarked for accuracy against Infinium arrays, RRBS, WGBS sequencing and locus specific-bisulfite sequencing performed on the same embryonic stem cell line. methylCRF transformation of MeDIP-seq/MRE-seq was equivalent to a biological replicate of WGBS in quantification, coverage and resolution.

This page describes how to download the methylCRF-suite of programs and run it on a MeDIP-seq/MRE-seq data set.

flashw简单加速器-快连加速器app

Name

Synapsis

Description

Arguments
小火箭免费节点公众号MeDIP-seq (with with uniq alignemnts)
MRE_seq.bedMRE-seq (normalized read counts by MRE_norm.pl)
小火箭收费节点在哪买CpG's sampled by MRE-seq. format: CpGid[0|1] where CpGid corresponds to cpg.bed.
Options
-gdirdirectory with model specific files: crf.list, cut.list, model files: [crfname].mdl [dflt: mdl]
-gdatdirectory with genomic specific files: cpg.bed (Epigenetic Roadmap blacklist removed), gdata.tbl, [crfnm]_cpg.bin [dflt: gdat]
-gapThe size of gap between consecutive CpG's for which to start a new region: 750bp was used for model in paper. [dflt: 750]
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-sfrom/-gtoOptionally only run certain steps (sfrom: start from, gto: go untill):
  1. Generate MRE/DIP avg window score files (1.5 DIPMRE count files)
  2. Shadowrocket配置V2Ray教程 – 月下博客:2021-3-14 · 小火箭添加节点 界面 4. 配置界面会随着协议类型改变，所众首先我伔要选择类型。点击“类型”进入协议选择界面： 小火箭选择协议见面 5. 小火箭支持非常多的类型，v2ray用的是”Vmess”协议，点击”Vmess”程序跳到Vmess配置界面 ...
  3. Generate CRF tables (~ 1.5 hr)
  4. Prediction
  5. Combine predictions into ensemble
  6. make dirs to put extra files in [have to give explicitely]

flashw简单加速器-快连加速器app

  1. a working Unix-like OS
  2. 小火箭免费节点公众号
  3. samtools
  4. pre-aligned MeDIP-seq and MRE-seq libraries in bam format

flashw简单加速器-快连加速器app

  1. Get methylCRF code by either:
    • git
      git clone http://bitbucket.org/mscse/methylcrf.git
    • or wget 
      wget http://bitbucket.org/mscse/methylcrf/get/fd50dc97b171.zip 
unzip fd50dc97b171.zip

  2. Run make to compile binaries and set up links 
    cd methylCRF
make
    3. *Note: stay in the methylCRF directory for the following steps

  3. Retrieve a methylCRF model
    • H1ES 
      wget http://gkx17h.wcbzw.com/h1es_mdl.tgz
tar -zxf h1es_mdl.tgz

  4. Retrieve species-specific data files
    • hg19 
      wget http://gkx17h.wcbzw.com/hg19/hg19_gdat.tgz
tar -zxf hg19_gdat.tgz
    • Please email to request other species not listed below.
  5. Prepare MeDIP-seq and MRE-seq data
    • Generate from sequencing experiments
      • 小火箭免费节点公众号
        • Generate sorted bed file of unique alignments. Note that the reads should be virtually extended to expected fragment length. The score field should 1.
        • ex: chr1 <begin> <begin+length> . 1 <strand>
      • MRE
        • Generate normalized MRE counts
        • Retreive an MRE fragment file for. Note for 5-enzyme MRE replace '3enz' with '5enz'. (email me for other species) 
              wget http://gkx17h.wcbzw.com/hg19/MRE_frags/MRE_3enz_4_6000.bed

        • Run MRE_norm.pl. It outputs <expr_prefix>_MRE.bed (as well as some diagnostic files)
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          NOTE: the call number is the number of bases that were cut off the read during sequencing (it's optional)
    • Alternately Download preprocessed H1ES data used for the paper: 
      wget http://gkx17h.wcbzw.com/h1es_dipmre.tgz
tar -zxf h1es_dipmre.tgz
  6. Estimate Methylation by running methylCRF
    • for example, for the H1ES data set used in the paper: 
      export PATH="~/src/methylCRF/:$PATH"
./methylCRF.pl -eid H1ES -mdir h1es_mdl -gdat hg19_gdat H1ESmrg_DIP.bed H1ESmrg_MRE.bed MRE_3enz_4_6000_cpg.bin >H1ES_mCRF.bed 2>err
      NOTE1: use the 3 or 5 enzyme fragment file use for MRE normalization.
      NOTE2: if some script can't find another, check that you have . in your path (ie, in: echo $PATH)

flashw简单加速器-快连加速器app

flashw简单加速器-快连加速器app

Experimental Data

Model

Species Data

flashw简单加速器-快连加速器app



flashw简单加速器-快连加速器app

Please post questions here or send questions directly to methylcrf _at_ googlegroups.com